PSFLab
A powerful yet easy-to-use numerical laboratory for calculating and visualizing the vectorial point spread function of optical microscopes
A powerful yet easy-to-use numerical laboratory for calculating and visualizing the vectorial point spread function of optical microscopes
PSFLab is a software program that calculates the illumination point spread function of a confocal microscope under various imaging conditions. It is based on rigorous vectorial theory and correctly accounts for various experimental parameters, such as light polarization, high numerical aperture objectives, and the effect of immersion medium, coverslip, and sample medium.
The underlying theory is described in the following journal article:
M. J. Nasse and J. C. Woehl
”Realistic modeling of the illumination point spread function in confocal scanning optical microscopy”
Journal of the Optical Society of America A 27, 295-302 (2010)
PSFLab is available in 64-bit for Windows (1024×768 screen or higher) and Mac (intel-based; 1280×800 screen or higher). A Linux version will soon be released as well.
The screenshots below are taken from version 3.0 – new images coming soon!